Specific Versus Non?specific Response in Exponential Molecular Amplification from Cross?Catalysis: Modeling the Influence of Background Amplifications on the Analytical Performances

Molecule based signal amplifications relying on an autocatalytic process may represent ideal strategy for the development of ultrasensitive analytical or bioanalytical assays, main reason being exponential nature amplification. However, to take full advantage such amplification rates, high stability starting co-reactants is required in order avoid any undesirable background Here, basis a simple kinetic model cross-catalysis including certain degree intrinsic instability co-reactants, we highlight key parameters governing response system and discuss performances that are expected from given set. In particular, show how detection limit directly related relative reactants within each catalytic loop. The validated with experimental dataset intended serve as guide design optimization molecular-based systems improved performances.